The assay time is considerably reduced from about an hour to few minutes by the newly reported RTF-EXPAR method which uses reverse transcriptase-free (RTF) approach for conversion of RNA í DNA followed by EXPAR (Exponential Amplification Reaction) for amplification at single temperature.
Controlling the rate of Covid-19 spread requires an accurate and a faster virus testing strategy. RT-PCR (reverse transcriptase polymerase chain reaction), the most accurate testing method being currently employed is a two-step test that takes around 60 minutes per sample.
Vísindamenn frá háskólanum í Birmingham hafa greint frá nýrri aðferð til að greina SARS-CoV-2. Þetta gæti gert mun hraðari prófun kleift og er nægilega viðkvæmt.
The veiru RNA uppgötvun af RT-PCR (öfug transkriptasa pólýmerasa keðjuverkun) felur í sér umbreytingu á veiru RNA í viðbót DNA (cDNA) fylgt eftir með mögnun á cDNA með magni PCR (qPCR). cDNA er síðan greint með því að nota flúrljómandi litarefni. Þetta tekur allt að klukkutíma.
The assay time is considerably reduced from about an hour to few minutes by the newly reported RTF-EXPAR method which uses reverse transcriptase-free (RTF) approach for conversion of RNA into DNA followed by EXPAR (Exponential Amplification Reaction) for amplification at single temperature. The amplification taking place at a single temperature is the key to speed, because it avoids lengthy heating and cooling steps of RT-PCR. Further, the section of DNA being amplified is smaller compared RT-PCR. Hence, EXPAR generates up to 108 strands of DNA product in few minutes. The duplex formation is monitored, as in RT-PCR method using fluorescent dye, SYBR Green.
Athyglisvert er að hægt er að breyta nýju aðferðinni til að greina nokkra aðra smitsjúkdóma af völdum RNA veira, til dæmis ebólu, RSV o.s.frv.
Heimildir):
Carter o.fl. (2020). Undir-5 mínútna uppgötvun á SARS-CoV-2 RNA með því að nota bakrita-frjáls veldisvísismögnunarviðbrögð, RTF-EXPAR. Forprentun. Birt á medRxiv Birt 04. janúar 2021. DOI: https://doi.org/10.1101/2020.12.31.20248236
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